Analytes & Panels

Luminex를 이용한 multiplex 분석 키트

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Analytes & Panels

Configurable-Human Cytokine Panel A 

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댓글 0건 조회 187회 작성일 24-05-09 17:03

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Target Analytes

Analyte Standard Curve(pg/mL) Serum Plasma CellCulture Sensitivity(pg/mL)
CCL2/JE/MCP-1 3.29 - 2,400 1:4 1:4 1:2 0.47
CCL3/MIP-1 alpha 49.4 - 12,000 1:4 1:4 1:2 28.3
CCL4/MIP-1 beta 5.76 - 4,200 1:4 1:4 1:2 0.72
CCL5/RANTES 2.33 - 1,700 1:100 1:100 1:2 1.91
CXCL5/ENA-78 5.49 - 4,000 1:4 1:4 1:2 4.14
FGF basic/FGF2/bFGF 8.59 - 2,200 1:4 1:4 1:2 1.82
G-CSF 3.29 - 2,400 1:4 1:4 1:2 1.48
GM-CSF 7.41 - 1,800 1:4 1:4 1:2 1.98
IFN-gamma 2.61 - 1,900 1:4 1:4 1:2 1.27
IL-1 alpha/IL-1F1 3.57 - 2,600 1:4 1:4 1:2 0.36
IL-1 beta/IL-1F2 2.63 - 1,920 1:4 1:4 1:2 0.57
IL-1ra/IL-1F3 16.9 - 4,100 1:4 1:4 1:2 10.91
IL-2 3.18 - 2,320 1:4 1:4 1:2 2.23
IL-4 9.05 - 2,200 1:4 1:4 1:2 4.46
IL-5 1.78 - 1,300 1:4 1:4 1:2 0.71
IL-6 3.29 - 2,400 1:4 1:4 1:2 1.11
IL-8/CXCL8 3.43 - 2,500 1:4 1:4 1:2 1.97
IL-10 3.18 - 2,320 1:4 1:4 1:2 0.3
IL-17/IL-17A 2.88 - 2,100 1:4 1:4 1:2 1.1
Thrombopoietin/Tpo 23 - 16,660 1:4 1:4 1:2 9.94
TNF-alpha 5.35 - 3,900 1:4 1:4 1:2 1.5
VEGF 0.586 - 2,400 1:4 1:4 1:2 0.81

* CCL5  requires at least a 1:100 dilution when assaying serum and plasma samples. 



특징 


  • 22가지 human cytokine중 원하는 타겟을 선정할 수 있는 multiplex performance kit
  • 소량의 샘플 이용 (<50ul)
  • 재현성 있는 결과를 위해 mass-calibrated standard 제공
  • 손쉬운 wash step을 위한 magnetic format 
  • R&D Systems의 Quantikine ELISA Kit와 비슷한 수준의 validation testing을 거쳐 생산됨


Kit components

  • Premixed cocktail of antibody-coated Magnetic beads
  • Premixed cocktail of biotinylated detection antibody
  • Standard Cocktail(s)
  • Bead Diluent
  • Biotin Antibody Diluent
  • Calibrator Diluent
  • Wash Buffer
  • Streptavidin-PE
  • One flat-bottom 96-well Microplate
  • Foil Plate Sealers (4)
  • Mixing Bottle
  • Standard Value Card (A standard curve must be generated each time an assay is run, utilizing values from the Standard Value Card included in the Kit.)



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