Analytes & Panels

Luminex를 이용한 multiplex 분석 키트

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Analytes & Panels

Configurable-Human High Sensitivity Cytokine Panel B 

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댓글 0건 조회 216회 작성일 24-05-09 17:10

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Target Analytes

Analyte Standard Curve(pg/mL) Serum Plasma CellCulture Sensitivity(pg/mL)
GM-CSF 1.6 - 6,555 1:2 1:2 1:2 0.155
IFN-gamma 1.9 - 7,685 1:2 1:2 1:2 0.25
IL-1 beta/IL-1F2 1 - 4,165 1:2 1:2 1:2 0.146
IL-2 1.26 - 5,155 1:2 1:2 1:2 0.386
IL-5 0.6 - 2,570 1:2 1:2 1:2 0.06
IL-6 0.7 - 2,800 1:2 1:2 1:2 0.135
IL-7 0.9 -3,620 1:2 1:2 1:2 0.14
IL-13 6.25 - 25,600 1:2 1:2 1:2 2.01
IL-15 0.6 - 2,350 1:2 1:2 1:2 0.167
IL-17/IL-17A 2.5 - 10,275 1:2 1:2 1:2 0.349
IL-22 2.34 -9,560 N/A N/A 1:2 0.529
IL-23 20.2 - 82,445 1:2 1:2 N/A 9.52
IL-31 4.5 - 18,505 1:2 1:2 1:2 0.117
IL-33 1.8 - 7,470 1:2 1:2 1:2 1.06
IL-36 beta/IL-1F8 0.5 - 2,035 1:2 1:2 1:2 0.25
TNF-alpha 1.1 - 4,435 1:2 1:2 1:2 0.25
IL-17F 9.2 - 37,640 1:2 1:2 1:2 4.54


* IL-17/IL-17A cannot be multiplexed with IL-17F

* Serum and plasma samples are not recommended for measurement of human IL-22




특징 


  • 17가지 human cytokine중 원하는 타겟을 선정할 수 있는 multiplex performance kit로 High Sensitivity (HS) 분석에 이용
  • 소량의 샘플 이용 (<50ul)
  • 재현성 있는 결과를 위해 mass-calibrated standard 제공
  • 손쉬운 wash step을 위한 magnetic format 
  • R&D Systems의 Quantikine ELISA Kit와 비슷한 수준의 validation testing을 거쳐 생산됨


Kit components

  • Premixed cocktail of antibody-coated Magnetic beads
  • Premixed cocktail of biotinylated detection antibody
  • Standard Cocktail(s)
  • Bead Diluent
  • Biotin Antibody Diluent
  • Calibrator Diluent
  • Wash Buffer
  • Streptavidin-PE
  • One flat-bottom 96-well Microplate
  • Foil Plate Sealers (4)
  • Mixing Bottle
  • Standard Value Card (A standard curve must be generated each time an assay is run, utilizing values from the Standard Value Card included in the Kit.)



 

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