Analytes & Panels

Luminex를 이용한 multiplex 분석 키트

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Configurable-Human Immuno-Oncology Panel

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댓글 0건 조회 233회 작성일 24-05-09 17:14

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Target Analytes
 
Analyte Standard Curve(pg/mL) Serum Plasma CellCulture Sensitivity(pg/mL)
CCL17/TARC 1.25 - 940 1:2 1:2 1:2 0.298
CD27/TNFRSF7 17.8 - 13,000 1:2 1:2 1:2 1.19
CD28 160 - 116400 1:2 1:2 1:2 21
CD40/TNFRSF5 4.3 - 3100 1:2 1:2 1:2 1.17
CD25/IL-2R alpha 2.8 - 2060 1:2 1:2 1:2 0.462
CTLA-4 52.3 - 38120 N/A N/A 1:2 8.61
CXCL5/ENA-78 18.8 - 13720 1:2 1:2 1:2 18
HVEM/TNFRSF14 53.9 - 39260 1:2 1:2 1:2 3.74
IFN-gamma 1.4 - 1010 1:2 1:2 1:2 0.294
LAG-3 240 - 174630 1:2 1:2 1:2 47.1
OX40 Ligand/TNFSF4 59.9 - 43660 1:2 1:2 1:2 10.8
PD-L1/B7-H1 2.3 - 1710 1:2 1:2 1:2 0.127
PD-L2/B7-DC 36.3 - 26490 1:2 1:2 1:2 2.44
TIM-3 3.8 - 2780 1:2 1:2 1:2 0.909

* CTLA-4 : Validated for use with cell culture supernates and cell lysates only.
* IFN-gamma is not valid when assaying Heparin.
* OX40L is not valid when assaying Heparin.




특징 


  • 14가지 human Immuno-Oncology 관련 biomarker 중 원하는 타겟을 선정할 수 있는 multiplex performance kit
  • 소량의 샘플 이용 (<50ul)
  • 재현성 있는 결과를 위해 mass-calibrated standard 제공
  • 손쉬운 wash step을 위한 magnetic format 
  • R&D Systems의 Quantikine ELISA Kit와 비슷한 수준의 validation testing을 거쳐 생산됨


Kit components

  • Premixed cocktail of antibody-coated Magnetic beads
  • Premixed cocktail of biotinylated detection antibody
  • Standard Cocktail(s)
  • Bead Diluent
  • Biotin Antibody Diluent
  • Calibrator Diluent
  • Wash Buffer
  • Streptavidin-PE
  • One flat-bottom 96-well Microplate
  • Foil Plate Sealers (4)
  • Mixing Bottle
  • Standard Value Card (A standard curve must be generated each time an assay is run, utilizing values from the Standard Value Card included in the Kit.)




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