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Fixed-Human Growth Factor Panel 21-plex
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Target Analytes
Analyte | Standard Curve(pg/mL) | Serum | Plasma | CellCulture | Sensitivity(pg/mL) |
CCL3/MIP-1 alpha | 13.7 - 3,340 | 1:2 | 1:2 | 1:2 | 1.58 |
CCL19/MIP-3 beta | 3.9 - 2,850 | 1:2 | 1:2 | 1:2 | 0.6 |
CD40 Ligand/TNFSF5 | 480 - 350,000 | 1:2 | 1:2 | 1:2 | 110.2 |
CXCL2/GRO beta/MIP-2/CINC-3 | 9.9 - 7,200 | 1:2 | 1:2 | 1:2 | 0.83 |
EGF | 6.7 - 4,880 | 1:2 | 1:2 | 1:2 | 1.21 |
FGF basic/FGF2/bFGF | 9.6 - 7,000 | 1:2 | 1:2 | 1:2 | 4.95 |
Flt-3 Ligand/FLT3L | 21.9 - 16,000 | 1:2 | 1:2 | 1:2 | 3.09 |
G-CSF | 9.4 - 6,880 | 1:2 | 1:2 | 1:2 | 0.62 |
Granzyme B | 7.5 - 5,500 | 1:2 | 1:2 | 1:2 | 1.04 |
IFN-beta | 2.7 - 2,000 | 1:2 | 1:2 | 1:2 | 0.31 |
IL-3 | 15.1 - 11,000 | 1:2 | 1:2 | 1:2 | 2.63 |
IL-8/CXCL8 | 1.8 - 1,300 | 1:2 | 1:2 | 1:2 | 0.36 |
IL-9 | 412 - 300,000 | 1:2 | 1:2 | 1:2 | 37.1 |
IL-17E/IL-25 | 17.8 - 13,000 | 1:2 | 1:2 | 1:2 | 1.95 |
IL-33 | 8.9 - 6,500 | 1:2 | 1:2 | 1:2 | 1.92 |
PD-L1/B7-H1 | 5.9 - 4,320 | 1:2 | 1:2 | 1:2 | 0.532 |
PDGF-AA | 6.6 - 4,800 | 1:10 | 1:2 | 1:2 | 0.86 |
PDGF-AB/BB | 6.9 - 5,000 | 1:10 | 1:2 | 1:2 | 0.23 |
TGF-alpha | 8.8 - 6,400 | 1:2 | 1:2 | 1:2 | 0.93 |
TRAIL/TNFSF10 | 24 - 17,500 | 1:2 | 1:2 | 1:2 | 2.32 |
VEGF | 6.4 - 4,700 | 1:2 | 1:2 | 1:2 | 1.17 |
특징
- 21가지 human growth factor를 동시에 분석할 수 있는 multiplex performance kit
- 소량의 샘플 이용 (<50ul)
- 재현성 있는 결과를 위해 mass-calibrated standard 제공
- 손쉬운 wash step을 위한 magnetic format
- R&D Systems의 Quantikine ELISA Kit와 비슷한 수준의 validation testing을 거쳐 생산됨
Kit components
- Premixed cocktail of antibody-coated Magnetic beads
- Premixed cocktail of biotinylated detection antibody
- Standard Cocktail(s)
- Bead Diluent
- Biotin Antibody Diluent
- Calibrator Diluent
- Wash Buffer
- Streptavidin-PE
- One flat-bottom 96-well Microplate
- Foil Plate Sealers (4)
- Mixing Bottle
- Standard Value Card (A standard curve must be generated each time an assay is run, utilizing values from the Standard Value Card included in the Kit.)
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- Fixed-Human Immunotherapy Panel 25-plex
- 24.05.09
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- Fixed-Human Chemokine Panel 8-plex
- 24.05.09
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