Analytes & Panels

Luminex를 이용한 multiplex 분석 키트

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Analytes & Panels

Fixed-Human XL Cytokine Panel 46-plex

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댓글 0건 조회 168회 작성일 24-05-09 16:51

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Target Analytes


Analyte Standard Curve(pg/mL) Serum Plasma CellCulture Sensitivity(pg/mL)
CCL2/JE/MCP-1 4.5 - 3,300 1:2 1:2 1:2 0.6
CCL3/MIP-1 alpha 13.7 - 3,340 1:2 1:2 1:2 1.58
CCL4/MIP-1 beta 82.3 - 20,000 1:2 1:2 1:2 18.4
CCL5/RANTES 247 - 180,000 1:2 1:2 1:2 40.7
CCL11/Eotaxin 31.6 - 23,000 1:2 1:2 1:2 1.81
CCL19/MIP-3 beta 3.9 - 2,850 1:2 1:2 1:2 0.6
CCL20/MIP-3 alpha 2.2 - 1,600 1:2 1:2 1:2 1.05
CD40 Ligand/TNFSF5 480 - 350,000 1:2 1:2 1:2 110.2
CXCL1/GRO alpha/KC/CINC-1 57.6 - 14,000 1:2 1:2 1:2 25.2
CXCL2/GRO beta/MIP-2/CINC-3 9.9 - 7,200 1:2 1:2 1:2 0.83
CXCL10/IP-10/CRG-2 2.5 - 1,800 1:2 1:2 1:2 0.16
EGF 6.7 - 4,880 1:2 1:2 1:2 1.21
FGF basic/FGF2/bFGF 9.6 - 7,000 1:2 1:2 1:2 4.95
Flt-3 Ligand/FLT3L 21.9 - 16,000 1:2 1:2 1:2 3.09
G-CSF 9.4 - 6,880 1:2 1:2 1:2 0.62
GM-CSF 16.5 - 12,000 1:2 1:2 1:2 1.56
Granzyme B 7.5 - 5,500 1:2 1:2 1:2 1.04
IFN-alpha 2/IFNA2 4.1 - 3,000 1:2 1:2 1:2 0.29
IFN-beta 2.7 - 2,000 1:2 1:2 1:2 0.31
IFN-gamma 0.5 - 380 1:2 1:2 1:2 0.062
IL-1 alpha/IL-1F1 9.9 - 7,200 1:2 1:2 1:2 0.41
IL-1 beta/IL-1F2 3.4 - 2,500 1:2 1:2 1:2 0.25
IL-1ra/IL-1F3 11 - 8,000 1:2 1:2 1:2 5.98
IL-2 3.4 - 2,500 1:2 1:2 1:2 0.25
IL-3 15.1 - 11,000 1:2 1:2 1:2 2.63
IL-4 1.1 - 800 1:2 1:2 1:2 0.07
IL-5 6.2 - 4,500 1:2 1:2 1:2 0.31
IL-6 8.6 - 6,300 1:2 1:2 1:2 0.38
IL-7 3.3 - 2,400 1:2 1:2 1:2 0.18
IL-8/CXCL8 1.8 - 1,300 1:2 1:2 1:2 0.36
IL-9 412 - 300,000 1:2 1:2 1:2 37.1
IL-10 61.1 - 44,560 1:2 1:2 1:2 5.47
IL-12 p70 18.5 - 13,500 1:2 1:2 1:2 2.39
IL-13 28.4 - 20,700 1:2 1:2 1:2 3.39
IL-15 2.3 - 1,700 1:2 1:2 1:2 0.26
IL-17/IL-17A 8.1 - 5,900 1:2 1:2 1:2 1.09
IL-17E/IL-25 17.8 - 13,000 1:2 1:2 1:2 1.95
IL-33 8.9 - 6,500 1:2 1:2 1:2 1.92
Lymphotoxin-alpha/TNF-beta 0.8 - 600 1:2 1:2 1:2 0.196
PD-L1/B7-H1 5.9 - 4,320 1:2 1:2 1:2 0.532
PDGF-AA 6.6 - 4,800 1:10 1:2 1:2 0.86
PDGF-AB/BB 6.9 - 5,000 1:10 1:2 1:2 0.23
TGF-alpha 8.8 - 6,400 1:2 1:2 1:2 0.93
TNF-alpha 11 - 8,000 1:2 1:2 1:2 0.62
TRAIL/TNFSF10 24 - 17,500 1:2 1:2 1:2 2.32
VEGF 6.4 - 4,700 1:2 1:2 1:2 1.17




특징 


  • 46가지 human cytokine을 동시에 분석할 수 있는 multiplex performance kit
  • 소량의 샘플 이용 (<50ul)
  • 재현성 있는 결과를 위해 mass-calibrated standard 제공
  • 손쉬운 wash step을 위한 magnetic format 
  • R&D Systems의 Quantikine ELISA Kit와 비슷한 수준의 validation testing을 거쳐 생산됨


Kit components

  • Premixed cocktail of antibody-coated Magnetic beads
  • Premixed cocktail of biotinylated detection antibody
  • Standard Cocktail(s)
  • Bead Diluent
  • Biotin Antibody Diluent
  • Calibrator Diluent
  • Wash Buffer
  • Streptavidin-PE
  • One flat-bottom 96-well Microplate
  • Foil Plate Sealers (4)
  • Mixing Bottle
  • Standard Value Card (A standard curve must be generated each time an assay is run, utilizing values from the Standard Value Card included in the Kit.)



 

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